Studies on glucose 6 phosphate g6p phosphate translocator isoforms gpt1 and gpt2 reported the viability of arabidopsis arabidopsis thaliana gpt2 mutants whereas heterozygous gpt1 mutants exhibited a variety of defects during fertilization seed set indicating that gpt1 is essential for this process.
Mobitec m el.
Among other functions gpt1 was shown to be important for pollen and embryo sac development.
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Negative controls were incubated either omitting the primary antibody or blocking the primary antibody with the respective peptide.
Voss m bel v100 kommode 473 breite.
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Nuclei were counterstained with 1 µg ml hoechst 33258 sigma aldrich munich germany.
By combining exm with single molecule localization.
Fisher hmsir 647 a208 01 mobitec conjugated to goat anti rabbit igg f ab 2 sab3700946 sigma tetraspeck microspheres 0 1 µm t27279 thermo fisher.
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Expansion microscopy exm enables super resolution fluorescence imaging of physically expanded biological samples with conventional microscopes.
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Slides were covered using mobiglow mounting medium mobitec goettingen germany and appropriate cover slips.